Technologies

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One-step COVID-19 colorimetric LAMP detection kit

Technology Overview

This technology is a simple one-step method that utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP) technique to amplify genetic materials of SARS-CoV-2, the causative of COVID-19, and xylenol orange colorimetric method to visualize the result as indicated by color change. This simple, rapid, and inexpensive test can be used for screening COVID-19 patients.

We are looking for international companies to license this technology.

Technology Features, Specifications and Advantages

This technology is simple, rapid, and inexpensive since it is designed to be seamless as much as possible. It contains a ready-to-use reagent, and thus only requires users to supply the test RNA into the reaction tubes prior to incubation at 65 ºC for 75 minutes. The LAMP method requires inexpensive heating block, which is 100 times cheaper than real -time PCR system. The presence of SARS-CoV-2 in the test samples will automatically trigger the change of reaction hues from purple to yellow. Importantly, the testing material cost of our kit is 3 times cheaper than the standard RT-qPCR.

Our kit has superb efficiency with the specificity, sensitivity, and accuracy of 100%, 92% (Limit of Detection (LOD) less than 4,000 copies/ml) and 97%, respectively. The result is shown in 75 min, which is twice as fast as an RT-PCR method.

Potential Applications

The primary application area of our technology is for screening COVID-19 patients. It is strictly to be used for in vitro diagnosis (IVD) for the purpose of rapid COVID-19 screening by trained laboratory technicians in certified laboratories.

Customer Benefit

  • 1.5X faster turnaround time compared to RT-PCR.
  • 3X cheaper compared to RT-PCR’s initial cost.
  • 60X cheaper compared to RT-PCR’s read-out.
  • 2X higher performance compared to RT-PCR.
OVERVIEW
Contact Person

Teeranit RATANAVARAHA

Organisation

National Science and Technology Development Agency

Technology Category

  • Healthcare
  • Diagnostics
  • Life Sciences
  • Biotech Research Reagents & Tools

Technology Readiness Level

Keywords

COVID-19 test kit, LAMP detection kit, COVID-19 screening