Alan J. Tackett, PhD
University of Arkansas for Medical Sciences
Professor of Biochemistry & Molecular Biology, Pediatrics and Pathology
Director UAMS Proteomics Facility
Director Arkansas INBRE Research Technology Core
Title: Approaches for Creating and Sustaining Core Facilities

My laboratory focuses on histone epigenetic mechanisms that regulate gene transcription and that are coupled to melanoma progression. We utilize a suite of techniques in our studies including proteomics of human biopsies, immunohistochemistry, cell culture, tumorigenicity assays, ChIPseq, biochemical and proteomic approaches for analyses of protein complexes, and cutting-edge mass spectrometry for the analysis of histone post-translational modifications. We have a unique environment that provides for discovery-based studies like proteomics as well as hypothesis-driven studies like those we perform in cell culture and with skin biopsies. In addition to our melanoma work, we develop new technologies for epigenetic studies such as tools for detection of in vivo protein interactions and quantitative assays for histone modifying proteins. I direct the UAMS Proteomics Facility and have extensive experience in collaborative research projects involving various areas of mass spectrometry. Furthermore, I serve as the Director of the Research Technology Core for the Arkansas INBRE grant, which utilizes a voucher system to fund cutting-edge research projects across the state.