2019 Molecular, Cellular and Tissue Bioengineering Symposium
8:30 am - 8:45 pm
SkySong 3 - Synergy Rooms
1365 N. Scottsdale Road
Scottsdale, AZ, 85257
Overview of the day

Single Cell Technologies from Method Development to Application

Single cell analysis is a vital part of understanding the variability in normal and diseased biological systems. The Molecular, Cellular, and Tissue Bioengineering (MCTB) faculty cluster at Arizona State University is excited to announce a symposium from experts in the single cell field and a hands-on single cell RNA-seq analysis training session on April 5th, 2019.

The symposium section from single cell experts will begin in the morning and finish in a networking event in the late afternoon (4 pm). The symposium will cover topics including:

  • Single cell technologies including RNA-seq and proteomics (CyToF)
  • Application of single cell analyses to cellular differentiation
  • Application to human disease with a focus on cancer
  • Future directions of single cell technologies and analysis approaches

The hands-on section single cell RNA-seq (scRNA-seq) analysis training session will continue following the symposium in the same room. The hands-on session will cover:

  • From FASTQ to count matrix (10X genomics cellranger)
  • Combining scRNA-seq datasets (Seurat and SCRAN)
  • Seurat scRNA-seq analysis suite of tools:
    • Data import, normalization, regressing out confounders, and scaling
    • Clustering
    • Visualization
    • Building a classifier to apply to other scRNA-seq datasets

The event will be held in the SKYSONG ASU Scottsdale innovation center. Lunch will be served and dinner for those attending the hands-on session.